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New amount was created toward suspended crushed products

New amount was created toward suspended crushed products

A couple of replicates off ten fresh fruit per readiness stage for every single duplicate were selected, ground, and you will leftover during the ?80°C having biochemical research, especially dissolvable sugars, normal acids, and you may erratic substances. Per simulate, ten ml out-of distilled water is put into 2.5 grams away from suspended surface apricot. The latest preparing is actually homogenized and you can centrifuged getting ten minute, at 4°C during the 9000 grams (Hettich 320R, Bach, Switzerland). New supernatant is actually retrieved after filtration toward stamen. Brand new supernatant need to be toned down in order to match the focus variety of this new enzymatic kits.

Glucose (sugar, fructose, and you will sucrose) and you can all-natural acids (malic and citric acids) was in fact quantified playing with an enthusiastic enzymatic strategy with establishes getting restaurants investigation (R-Biopharm AG, Darmstad, Germany), especially specific enzymatic decide to try sets having L-malic acidic, citric acidic, glucose/fructose, and you will sucrose.